This elastase assay works under a variety of conditions. Perform your assays in
solution or in cell suspension. Even under the microscope!
For example, one application is screening for anti-inflammatory drug candidates using a
fluorescent plate reader. The measured Km of this substrate with porcine pancreatic
elastase is in the 800 nM range, in contrast to the Km values of 6.2 and 0.8 mM for other
chromogenic substrates based on pNA and AMC chromophores, respectively [see M.J. Castillo,
K. Nakajima, M. Zimmerman and J.C. Powers Anal Biochem 99:53-64 (1979)].
This is truly an example of technology-driven science, when you consider that elastase
activity can now be measured under conditions you did not think possible.
No other elastase assay is like it!
Sensitive- with Km at 800 nM- this is fluorescence, after all
Broadly applicable - clear solutions, serum, cell suspensions, or on
a microscope slide
You can also use these fluorogenic substrates for:
- flow cytometric analysis
- histology frozen sections
Application notes with supporting data will soon be available
If your needs are not met by the above, just contact us!