Cell-Mediated Cellular Cytotoxicity

 

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GranToxiLux® and PanToxiLuxTM

Live, Single Cell-based Fluorogenic Cytotoxicity Assay Kits
for Measuring CTL or NK Cell-Mediated Cytotoxic Activity

A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte

 

Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:

GranToxiLux®: granzyme B
PanToxiLuxTM: granzyme B and caspase-8

Measurements with GranToxiLux® and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).  In the figure below, CTL responses are detected with OncoImmunin's first generation cell- mediated cytotoxicity kit, CyToxiLux®.  Earlier events, e.g., delivery of granzyme B and/or caspase-8 from CTLs into target cells, are now detectable by GranToxiLux® or PanToxiLuxTM.

 

Selected References
J. Immunology 179:3812-20 (2007); Immunity 29:1009-21 (2008) (Preview: 845-7); Natural Killer Cells ed. M.T. Lotze and A.W. Thomson, 645- 652 (2010); PLoS One 10:e0145249 (2015); AIDS Res Hum Retroviruses 32:1079-1088 (2016); Front Immunol. 10:735 (2019)

 

 

Detailed GranToxiLux PLUS! instructions are available for download in PDF format
Detailed PanToxiLuxTM instructions are available for download in PDF format
Detailed PanToxiLux P2D2 instructions are available for download in PDF format
NEW - prior to cytotoxicity assay, eliminate dead target cells with NFL1

 

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Live, Single Cell-based Fluorogenic Cytotoxicity Assay Kits
for Measuring Antibody-Dependent Cellular Cytotoxicity (ADCC)

Selected References
J. Immunology 179:3812-20 (2007); Immunity 29:1009-21 (2008) (Preview: 845-7); Natural Killer Cells ed. M.T. Lotze and A.W. Thomson, 645- 652 (2010); PLoS One 10:e0145249 (2015); AIDS Res Hum Retroviruses 32:1079-1088 (2016); Front Immunol. 10:735 (2019)

 

 

Detailed GranToxiLux PLUS! instructions are available for download in PDF format
Detailed PanToxiLuxTM instructions are available for download in PDF format
Detailed PanToxiLux P2D2 instructions are available for download in PDF format
NEW - prior to cytotoxicity assay, eliminate dead target cells with NFL1

 

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OncoImmunin, Inc.,
207A Perry Parkway, Suite 6
Gaithersburg, MD 20877
Phone: (301) 987-7881
FAX: (301) 987-7882
email:info@PhiPhiLux.com

 

 

 

 

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