|
| The Objective: A molecular design principle for: |
The Solution: |
 | Peptide substrates for proteases associated with apoptosis, metastasis, inflammation,
and viral replication |
|
A new class of cell and tissue
permeable fluorogenic protease substrates
that utilize intramolecular fluorophore dimers for fluorescence and
absorption measurements |
|
| Diagnostics for apoptosis, metastasis, inflammation and viral
replication |
|
| Cell and tissue permeable inhibitors for apoptosis, metastasis,
inflammation and viral replication |
|
Modification
of OncoImmunin's design for cell and tissue permeable substrates:
substitution of a noncleavable bond at protease recognition sites |
| Drug delivery vehicles |
|
Use of OncoImmunin's substrate
design which allows cell and tissue permeability |
OncoImmunin, Inc. recently designed and synthesized a new class of fluorogenic
protease substrates for enzymatic activity determination in serum as well as whole
cells. References are available.
The advantages of OncoImmunin's protease substrates over other
fluorogenic substrates are
4-fold:
| Fluorescent probes were selected to ensure accurate enzymatic activity
determination not only in solution, but in biological samples such as serum,
live cells, and tissues.
|
| Intracellular protease activity can be visualized using a standard fluorescence
microscope- without any modification!
|
| To ensure specificity, all substrates contain amino acid sequences from both
sides of cleavage sites, i.e. from Pn through Pn'.
|
| Substrates assume loop conformations in solution (see computer modeled structure
under principles) which mimic active site loop conformations of
naturally occurring target molecules.
|
OncoImmunin's fluorogenic protease substrate design allows determination of intracellular
enzymatic activity using a fluorescence microscope. Determination of activity in buffer
solution as well as in human serum can be accomplished using a standard fluorometer, or
for large sample throughput, a proprietary high-throughput screening (HTS)
system is available (ref the page)Note- Absorption measurements are also possible.
To learn more about OncoImmunin, Inc. and its enzymatic activity assay kits choose from a
category below:
|
