Cell-Mediated Cellular Toxicity

 

 

GranToxiLux® and PanToxiLux


Live, Single Cell-based Fluorogenic Cytotoxicity Assay Kits

A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte

Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:

GranToxiLux®: granzyme B
PanToxiLux™: granzyme B and caspase-8

 

Measurements with GranToxiLux® and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).  In the figure below, CTL responses are detected with OncoImmunin's first generation cell-mediated cytotoxicity kit, CyToxiLux®.  Earlier events, e.g., delivery of granzyme B and/or caspase-8 from CTLs into target cells, are now detectable by GranToxiLux® or PanToxiLuxTM.

Detailed GranToxiLux® PLUS! instructions are available for download in PDF format
Detailed PanToxiLux® instructions are available for download in PDF format
Detailed PanToxiLux™ P2D2 instructions are available for download in PDF format

NEW - prior to cytotoxicity assay, eliminate dead target cells with NFL1

rainbow line

OncoImmunin, Inc.,
207A Perry Parkway, Suite 6
Gaithersburg, MD 20877
Phone: (301) 987-7881
FAX: (301) 987-7882
email:info@PhiPhiLux.com

 

 

 

 

Copyright © 1998-2019 OncoImmunin, Inc. All rights reserved.