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CyToxiLux®
,
GranToxiLux®, and
PanToxiLux™
Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits
Principle of assays:
Following the successful delivery of a
lethal hit by cytotoxic lymphocytes, protease activities leading to cell death
in individual target cells can be measured by flow cytometry or fluorescence
microscopy. In each kit cleavage of a cell permeable fluorogenic substrate
reports the following activities:
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CyToxiLux® : downstream
caspase |
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GranToxiLux®: granzyme B |
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PanToxiLux™: granzyme B and upstream caspase |
Measurements with CyToxiLux®,
GranToxiLux®, and
PanToxiLuxTM are superior to bulk assays e.g., LDH and
51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity
(relatively weak CTL responses against subdominant epitopes are detectable).
Detailed CyToxiLux® PLUS! instructions are
available for download in PDF format
Detailed GranToxiLux® PLUS!
instructions are available for download in PDF format
Detailed GranToxiLux®
G2D2
instructions are available for download
in PDF format
Detailed PanToxiLux® instructions are
available for download in PDF format
NEW - prior to cytotoxicity assay, eliminate dead target cells with
NFL1
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